Lpl Knockout cell line （BV2）

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location： Home > Products > KO Cell Lines > Lpl Knockout cell line （BV2）

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Lpl Knockout cell line （BV2） Order

Lpl Knockout cell line （BV2）
Catalog#
YKO-M201
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Lpl Knockout cell line （BV2）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Lpl Knockout cell line (BV2)
Morphology
Epithelial-like,?adherent
Passage ratio
1:3~1:5
Species
Mouse
Gene Name
Lpl
Gene ID
16956
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Lpl-201)
Validation: PCR+Sanger Sequencing
Lpl Knockout cell line （BV2） Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Lpl gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BV2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Lpl gene knockout Lpl Knockout cell line (BV2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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